Are Western Blots Quantitative?

Western blot is a reliable quantitative method only if sample properties and integrity, antibody specificity to the target protein, and loading protocols are considered. With careful attention to details, you can avoid common mistakes and avoid misinterpreting Western blot data.

Why is western blot semi quantitative?

It is very important to be aware that the data produced with a western blot is typically considered to be semi-quantitative. This is because it provides a relative comparison of protein levels, but not an absolute measure of quantity.

How is western blot quantified?

Step 1: Determine the background-subtracted densities of your protein of interest (PI) and the normalizing control (NC). Step 2: Identify the NC that has the highest density value. Step 3: Divide all the NC values by the highest NC density value to get a relative NC value.