By Flow Cytometry and Cell Sorting?

The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. ...

Can flow cytometry sort cells?

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.

How are the cells sorted using flow cytometry?

Cells stained with fluorescently labeled antibodies enter the flow cell where hydrodynamic focusing lines them up single file, as they pass through a beam of laser light. The laser light is scattered by the cell and forward and side scatter are detected, representing cell size and complexity, respectively.