By Heparin Affinity Chromatography?

Heparin chromatography is an adsorption chromatography in which biomolecules can be specifically and reversibly adsorbed by heparins immobilized on an insoluble support. An advantage of this chromatography is that heparin-binding proteins can be conveniently enriched using its concentration effect.

What does a heparin column do?

Heparin column is sufficient to remove DNA from your protein because it serves as cation exchanger, charge repulsion with in the column will make DNA come down.

Why do DNA binding proteins have an affinity for heparin?

In its interaction with DNA binding proteins heparin mimics the polyanionic structure of the nucleic acid. In its interaction with coagulation factors such as antithrombin III, heparin acts as an affinity ligand.