Can Confocal Microscopy Work?

As a distinctive feature, confocal microscopy enables the creation of sharp images of the exact plane of focus, without any disturbing fluorescent light from the background or other regions of the specimen. Therefore, structures within thicker objects can be conveniently visualized using confocal microscopy.

Is confocal microscopy difficult?

It is preferable to do this survey using the microscope of the confocal system, but it can be extremely difficult for the novice to find the correct focal plane using the confocal imaging mode alone.

What are the limitations of confocal microscopy?

Disadvantages of confocal microscopy are limited primarily to the limited number of excitation wavelengths available with common lasers (referred to as laser lines), which occur over very narrow bands and are expensive to produce in the ultraviolet region (56).