Can Kakashi Use Susanoo?

During the battle with Kaguya, Obito lent Kakashi the full power of the Mangekyo Sharingan which gave him the ability to use Susanoo.

How could Kakashi use Susanoo?

Madara's moon plan was to reflect his eye to cast infinite Tsukuyomi. That means he is able to use Tsukuyomi in one of his eyes, which makes sense as he can use Susanoo also.

Can Susanoo be use without Sharingan?

The only logical conclusion is that after someone uses Susanoo, they can use it again without the Mangekyou Sharingan. The manga never explicitly stated that Susanoo required the usage of the MS. Rather it stated that Susanoo is the third technique MS users obtain after Tsukuyomi and Amaterasu.