Can Reverse Transcriptase Use Dna as a Template?

Reverse transcriptase first transcribes a complementary strand of DNA to make an RNA:DNA hybrid. Next, reverse transcriptase or RNase H degrades the RNA strand of the hybrid. The single-stranded DNA is then used as a template for synthesizing double-stranded DNA (cDNA).

Does reverse transcriptase work on DNA?

Molecular biology

The classical PCR technique can be applied only to DNA strands, but, with the help of reverse transcriptase, RNA can be transcribed into DNA, thus making PCR analysis of RNA molecules possible. Reverse transcriptase is used also to create cDNA libraries from mRNA.

Can reverse transcriptase use mRNA as a template?

(b) Second, the enzyme reverse transcriptase synthesizes strands of DNA using the mRNA molecules as templates.