During Which Phase Do They Separate?

Metaphase leads to anaphase, during which each chromosome's sister chromatids separate and move to opposite poles of the cell. Enzymatic breakdown of cohesin — which linked the sister chromatids together during prophase — causes this separation to occur.

During which phase does the cell actually separate?

Explanation: Cytokinesis is the phase during which the cytoplasm of the cell divides and the cell actually separates into two cells.

What phase of mitosis do they separate?

Anaphase is the fourth phase of mitosis, the process that separates the duplicated genetic material carried in the nucleus of a parent cell into two identical daughter cells.