How Are Embryonic Stem Cells Derived?

Embryonic stem cells are obtained from early-stage embryos — a group of cells that forms when a woman's egg is fertilized with a man's sperm in an in vitro fertilization clinic. ... The National Institutes of Health created guidelines for human stem cell research in 2009.

How do you extract embryonic stem cells?

Embryonic stem cells are usually harvested shortly after fertilization (within 4-5 days) by transferring the inner cell mass of the blastocyst into a cell culture medium, so that the cells can be multiplied in a laboratory.

How are hESC obtained?

hESC are derived from the inner cell mass of an embryonic blastocyst five days after fertilization.