How Big Is mCherry

Structure. The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa.

What is the size of GFP?

GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.

Is mCherry far red?

mCherry is an excellent fluorescent protein that has maximal emittance in the red spectral range and a high fluorescence quantum yield, and thus, can be used as a donor for energy transfer to a far-red acceptor, such as BDFP1. … 6, which resulted in a highly bright far-red fluorescent protein, BDFP2.