How Coagulase Test Is Done?

Procedure and Types of Coagulase Test
Add a drop of human or rabbit plasma to one of the suspensions, and mix gently. Look for clumping of the organisms within 10 seconds. No plasma is added to the second suspension to differentiate any granular appearance of the organism from true coagulase clumping.

What reagent is used for coagulase test?

Rabbit Coagulase Plasma is a standardized, lyophilised rabbit plasma used for the qualitative detection of the coagulase enzyme produced by Staphylococcus aureus.

What media is used to detect coagulase production?

Coagulase production can be detected using either the slide coagulase test (SCT) or the tube coagulase test (TCT). Slide coagulase detects bound coagulase (also called "clumping factor") [9], which reacts directly with fibrinogen in plasma, causing rapid cell agglutination.

Sophia Al-Mansoor

Sophia Al-Mansoor

Global Business & E-Commerce Reporter

Sophia analyzes international trade, startup ecosystems, retail transformation, and supply chain logistics for modern digital publications.