How Do You Make Nutrient Broth
Add 13G of Nutrient Broth Powder (Cm0001B) in 1L of Distilled Water. Mix and Dissolve Them Completely. Pour Them into the Final Containers (Eg. Conical...
Add 13g of nutrient broth powder (CM0001B) in 1L of distilled water.Mix and dissolve them completely.Pour them into the final containers (eg. conical flask)Sterilize by autoclaving at 121°C for 15 minutes.
How is nutrient broth prepared?
Suspend 8 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.
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What is the composition and uses of nutrient broth?
IngredientsAmount (gm/L)Peptone5.0 gmSodium chloride8.0 gmAgar15.0 gmDistilled water1000 mL
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