How Does a Fluorometer Work?

A fluorometer is a device that measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when one wavelength of light hits and excites electrons in a material, and the electrons in that object instantaneously emit (fluoresce) light of a different wavelength.

What is the principle of fluorometer?

Fluorophores absorb light of a distinct excitation wavelength and emit, or fluoresce, light of reduced energy thus a longer wavelength. This behavior can be modified so that the fluorescent reagents are restricted from emitting light unless bound to a specific molecule, such as dsDNA.

How does a Marine fluorometer work?

How does a fluorometer work? Fluorescence is the phenomenon of some compounds to absorb specific wavelength of light and almost instantaneously emit longer wavelengths of light. ... A fluorometer transmits an excitation beam of light in the blue range and detects the fluorescent red light emitted by the chlorophyll.