How Does Fluorescein Work in the Eye

You are asked to blink. Blinking spreads the dye and coats the tear film covering the surface of the cornea. The tear film contains water, oil, and mucus to protect and lubricate the eye. The health care provider then shines a blue light at your eye.

How does fluorescein stain your eyes?

Fluorescein staining of the cornea is performed by first placing a drop of sterile saline on a sterile fluorescein strip. The fluorescein is then placed in the inferior cul de sac of the eye by pulling down on the lower lid and gently touching the bulbar conjunctiva with the fluorescein strip.

Why does fluorescein stain the cornea?

Historically, sodium fluorescein staining has been thought to be a result of one of three mechanisms2: pooling in areas of shed cells, ingress around cells due to loss of tight junctions, or in dead or desquamating cells.

Robert Thorne

Robert Thorne

Automotive & Future Transportation Editor

Robert Thorne covers electric vehicle innovations, autonomous driving systems, global mobility trends, and automotive engineering developments.

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