How Does Microspectrophotometry Work?

How Microspectrophotometer Works. The microscope spectrometer and microspectrometer work like this: a lamp on the microscope emits white light which is focused onto the sample. ... The light not absorbed is collected by the microspectrophotometer objective and focused onto the entrance aperture of the spectrophotometer.

Why is Microspectrophotometry useful to a forensic technician?

In forensic science, MSP is most commonly used to measure and compare the color of single fibers. This can be done using unpolarized light or plain polarized light (to study pleochroism). Paints, minerals, and a variety of other trace evidence can also be studied.

Does UV VIS destroy sample?

Unfortunately, these processes destroy a sample, require large amounts of a sample, and suffer from the inaccuracies of the human visual system. But UV-VIS-NIR microspectrophotometers can analyze microscopic samples without destruction and can detect sample variations that would not be apparent by eye.