How Giemsa Stain Works?
Giemsa Solution Is Composed of Eosin and Methylene Blue (Azure). the Eosin Component Stains the Parasite Nucleus Red, While the Methylene Blue Component Stains...
Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue. The thin film is fixed with methanol. De-haemoglobinization of the thick film and staining take place at the same time.
What is the principle of Giemsa staining?
PRINCIPLE: The “neutral” dyes combining the basic dye methylene blue and the acid dye eosin, give a wide color range when staining. The pH of the staining solution is critical and ideally should be adjusted for different fixatives.
How long does it take to stain Giemsa?
The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
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