How Is Thromboplastin Produced?

They are manufactured using recombinant human tissue factor produced in Escherichia coli and synthetic phospholipids, which do not contain any other clotting factors such as prothrombin, factor VII and factor X.

What is the source of thromboplastin?

Historically, thromboplastin was a lab reagent, usually derived from placental sources, used to assay prothrombin times (PT). When manipulated in the laboratory, a derivative could be created called partial thromboplastin. Partial thromboplastin was used to measure the intrinsic pathway.

When is thromboplastin formed?

Upon the introduction of cells, particularly crushed or injured tissue, blood coagulation is activated and a fibrin clot is rapidly formed. The protein on the surface of cells that is responsible for the initiation of blood clotting is known as tissue factor, or tissue thromboplastin.