How to Equilibrate Hplc Column?
Equilibration Time Is Dependent on the Flow Rate and Dimension of the Column. in General, Flushing the Column with Ten up to Twenty Column Volumes Is Enough...
Equilibration time is dependent on the flow rate and dimension of the column. In general, flushing the column with ten up to twenty column volumes is enough for equilibration. Therefore the flow rate has to be kept in mind.
How long does it take to equilibrate a column?
Whereas the rule of thumb is that 10 column volumes of mobile phase is sufficient to equilibrate a column when switching from mixtures of water or buffer and acetonitrile or methanol, it may take 20–50 column volumes, or more, to equilibrate ion-pairing conditions.
How do I flush a new HPLC column?
In your case wash the column with 70% water; 15% methanol; 15% acetonitrile. Divert the column eluent to waste not to contaminate your detector(s). Wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes.
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