In Fluorometry the Emission Radiation Is Always?

The emitted radiation is always of a longer wavelength (lower energy) than the excitation radiation (higher energy). For example, if the incoming light was blue (shorter wavelength), then the appropriate fluorophore will emit green light (longer wavelength).

What is emission in fluorescence?

Emission spectra. Fluorescence emission behaves in a similar way: the fluorescence output of a fluorophore is most likely to occur at a particular wavelength. This wavelength is the emission maximum for that fluorophore. The excited fluorophore can also emit light at wavelengths near the emission maximum, as shown.

Why is emission wavelength longer than excitation?

When electrons go from the excited state to the ground state (see the section below entitled Molecular Explanation), there is a loss of vibrational energy. As a result, the emission spectrum is shifted to longer wavelengths than the excitation spectrum (wavelength varies inversely to radiation energy).