In Glc the Stationary Phase Is?
A Liquid Such as Silicone Grease Supported on an Inert Granular Solid, and the Mobile Phase Is an Inert Gas (N, He, Ar). Since Amino Acids Are Not Volatile...
In GLC, the stationary phase is a liquid such as silicone grease supported on an inert granular solid, and the mobile phase is an inert gas (N, He, Ar). Since amino acids are not volatile, they must be converted to volatile derivatives before analysis.
What is the stationary phase in chromatography?
Chromatography is a separation process involving two phases, one stationary and the other mobile. Typically, the stationary phase is a porous solid (e.g., glass, silica, or alumina) that is packed into a glass or metal tube or that constitutes the walls of an open-tube capillary.
What are the mobile and stationary phase used in GLC?
The mobile phase (=carrier gas) is comprised of an inert gas i.e., helium, argon, or nitrogen. The stationary phase consists of a packed column in which the packing or solid support itself acts as stationary phase, or is coated with the liquid stationary phase (=high boiling polymer).