On What Principle Is Spectrophotometry Based?

5: Spectrophotometry. Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength.

What is the principle of UV spectrophotometry?

UV Spectroscopy uses ultraviolet light to determine the absorbency of a substance. In simple terms, the technique maps the interaction between light and matter and measures. As matter absorbs light it undergoes either excitation or de-excitation, which generates what is known as a spectrum.

What is spectrophotometry used for?

Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength. The instrument used is called a spectrophotometer.