Sql Query with Not Like In

That's because you're mixing two syntax together.

If you always have exactly those three values, you can just AND the results of three LIKE expressions.

SELECT
  *
FROM
  Table1
WHERE
      EmpPU NOT LIKE '%CSE%'
  AND EmpPU NOT LIKE '%ECE%'
  AND EmpPU NOT LIKE '%EEE%'

If you need to do it for "any number" of values, you can put the values into a table and do a join.

WITH
  myData
AS
(
            SELECT '%CSE%' AS match
  UNION ALL SELECT '%ECE%' AS match
  UNION ALL SELECT '%EEE%' AS match
)

SELECT
  *
FROM
  Table1
LEFT JOIN
  myData
    ON Table1.EmpPU LIKE myData.match
WHERE
  myData.match IS NULL

OR...

WITH
  myData
AS
(
            SELECT '%CSE%' AS match
  UNION ALL SELECT '%ECE%' AS match
  UNION ALL SELECT '%EEE%' AS match
)

SELECT
  *
FROM
  Table1
WHERE
  NOT EXISTS (SELECT * FROM myData WHERE Table1.EmpPU LIKE match)

If you have set of words which you want to include/exclude in search from a particular column. You may want to use regular expression function of mysql.

Exclude set of words from a column :

SELECT
  *
FROM
  Table1
WHERE
      EmpPU NOT REGEXP 'CSE|ECE|EEE';

Search set of words from a column :

SELECT
  *
FROM
  Table1
WHERE
      EmpPU REGEXP 'CSE|ECE|EEE';

you cant combine LIKE and IN

you can do:

select * from Table1
where EmpPU not in ('%CSE%', '%ECE%', '%EEE%')

but you wont benefit from the % wildcard

if you need the % the only option is:

Select * from Table1
where EmpPU not like '%CSE%' and  EmpPU not like '%ECE%' and EmpPU not like '%EEE%'

Or you can do it like this:

SELECT 
    * 
FROM 
    Table1
WHERE NOT EXISTS
    (
        SELECT
            NULL
        FROM
        (
            SELECT '%CSE%' AS column1 UNION ALL 
            SELECT '%ECE%' UNION ALL 
            SELECT '%EEE%'
        ) AS tbl
        WHERE Table1.EmpPU LIKE tbl.column1
    )

you can try this

Select * from Table1 where 
    EmpPU NOT Like '%CSE%'  
AND EmpPU NOT Like '%ECE%' 
AND EmpPU NOT Like '%EEE%'

Code is as below:

Select * from Table1 where 
        (EmpPU NOT Like '%CSE%'  
    OR EmpPU NOT Like '%ECE%' 
    OR EmpPU NOT Like '%EEE%')

Or use EXCEPT:

    SELECT * FROM Table1 
    EXCEPT
    SELECT * FROM Table1 
    WHERE EmpPU LIKE '%CSE%'  
       OR EmpPU LIKE '%ECE%' 
       OR EmpPU LIKE '%EEE%'