What Are the Factors Affecting Pipetting Performance?
Temperature. Temperature Has Many Effects on Pipetting Accuracy. Density. the Density (Mass/Volume Ratio) Affects the Liquid Volume That Is Aspirated into the...
- Temperature. Temperature has many effects on pipetting accuracy.
- Density. The density (mass/volume ratio) affects the liquid volume that is aspirated into the tip.
- Altitude. Geographic altitude affects accuracy through air pressure.
How does temperature affect pipetting?
The warming of the pipette tip causes the captive air to expand and push liquid from the tip. Less liquid is aspirated and subsequently dispensed, leading to a variation between the target and delivered volumes. ... The captive air volume shrinks, causing more liquid to be aspirated and subsequently dispensed.
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Does surface tension affect pipettes?
The effect of a lowered surface tension can be reduced by slowing the pipetting speed. Additionally, the liquid retention effect can be reduced by using the reverse pipetting technique.
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