What Are the Interfering Substances Using Jaffe Reaction?
Chemical Methods Are Mostly Based on the Reaction of Creatinine with Alkaline Picrate (Jaffe Reaction). the Jaffe Reaction Is Not Specific for Creatinine, the...
.
Then, what is Jaffe method?
The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.
One may also ask, how does bilirubin affect creatinine? CONCLUSION: This shows that the bilirubin has negative interference in creatinine value measurement by ordinary laboratory practices and interference increases with higher concentration of bilirubin in blood sample. Creatine is present as creatine phosphate, reserved energy in muscle.
what is the principle of creatinine test?
The method is based on the Jaffe reaction. Creatinine reacts with picrate ion formed in alkaline medium to develop a red-orange colour. The colour produced from the sample is then compared in a colorimeter at wavelength of 505 nm with that produced by a known amount of creatinine under the same condition.
How the concentration of creatinine can be detected in blood?
Creatinine is a waste product produced by muscles from the breakdown of a compound called creatine. Creatinine is removed from the body by the kidneys, which filter almost all of it from the blood and release it into the urine. This test measures the amount of creatinine in the blood and/or urine.
