What Does an Isosbestic Point Indicate

* An isosbestic point is a value of frequency where the two species in an A ⇌ B equilibrium have the same absorption. … The presence of isosbestic points thus indicates the presence of only two species in equilibrium.

What is an isosbestic point when are Isosbestic points observed and why?

An isosbestic point is observed in overlaid spectra when a chromophoric precursor is converted to a product with a different spectrum, so that it is often assumed that an isosbestic point occurs only when the precursor is quantitatively converted to a single product.

Does the absorbance at the isosbestic point depend on indicator concentration or PH?

An isosbestic point is defined as the wavelength where two species have the same molar absorptivity. At the isosbestic point the total absorbance of a solution of the two ions is independent of their relative concentrations but is dependent only upon the total dye concentration.