What Does Each Diff-Quik Stain Do

The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.

What are the Diff-Quik stains?

The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.

How often should you change Diff-Quik stain?

Good laboratory practice should document changing each Diff-Quik stain setup at regular intervals (for example, every week if there is an average of about five evaluations per week). For immediate evaluation on wet fixed samples, an immersion stain setup could pose some threat of cross-contamination.