What Is a Urease Test Used For
The Urease Test Identifies Those Organisms That Are Capable of Hydrolyzing Urea to Produce Ammonia and Carbon Dioxide. It Is Primarily Used to Distinguish...
The urease test identifies those organisms that are capable of hydrolyzing urea to produce ammonia and carbon dioxide. It is primarily used to distinguish urease-positive Proteeae from other Enterobacteriaceae.
What is the positive result of urease test?
The urease produced by H. pylori hydrolyzes urea to ammonia, which raises the pH of the medium, and changes the color of the specimen from yellow (NEGATIVE) to red (POSITIVE).
How is urease test performed?
- Prepare an Urea broth by dissolving 2.95g of urea powder in 150ml of distilled water. Add urea after autoclaving the media to prevent urea from initial breakdown.
- Inoculate the given sample of organism aseptically using wire loop.
- Incubate the tubes at 37°C for 24 hours.
- Observe the result.
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