What Is Isopycnic Centrifugation?

Definition: A method where the components of a sample (e.g. DNA) are separated on the basis of their density in a centrifuge according to the centrifugal force they experience.

What is isopycnic condition?

Upon centrifugation, particles of a specific density sediment until they reach the point where their density is the same as the gradient media (i.e., the equilibrium position). The gradient is then said to be isopycnic and the particles are separated according to their buoyancy.

What is isopycnic banding in isopycnic separation?

In isopycnic centrifugation, using a continuous gradient, a fairly homogeneous population of organelles will 'band' in the gradient at their actual buoyant density over several hours. This method of separation is independent of time, and relies solely on the actual buoyant density of the particle.