What Is Liver Homogenate?

For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm. The word homogenate was introduced by VR Potter in1941 (J. Biol.

How do I make my liver homogenate?

What is the right method to get liver homogenate?
  1. put liver in a glass homogenizer and add nidefold volume of 0.25M sucrose solution(with 1mM EDTA)(10% W/V)
  2. homogenize liver and move 2ml of homogenate into 2ml eppendorf tube.
  3. do centrifuging at 700x g for 10 min to separate debris and nuclear.

What does homogenate mean in biology?

An unfractionated, cell-free, preparation of tissue prepared by disruption of tissue structure and breakage of cell walls, e.g. with a Potter-Elvehjem homogenizer or by use of ultrasonic vibrations.

David Miller

David Miller

Executive Financial & Market Analyst

David Miller brings 15 years of experience in global economics, personal finance strategy, and market dynamics. He specializes in turning complex economic trends into actionable insights for everyday readers.