What Is Liver Homogenate?
For Example, a Liver Homogenate Is a Liver Tissue in Which Its Cells Have Been Mechanically Disrupted, Releasing the Organelles and Cytoplasm. the Word...
For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm. The word homogenate was introduced by VR Potter in1941 (J. Biol.
How do I make my liver homogenate?
What is the right method to get liver homogenate?
- put liver in a glass homogenizer and add nidefold volume of 0.25M sucrose solution(with 1mM EDTA)(10% W/V)
- homogenize liver and move 2ml of homogenate into 2ml eppendorf tube.
- do centrifuging at 700x g for 10 min to separate debris and nuclear.
What does homogenate mean in biology?
An unfractionated, cell-free, preparation of tissue prepared by disruption of tissue structure and breakage of cell walls, e.g. with a Potter-Elvehjem homogenizer or by use of ultrasonic vibrations.