What Is Normal and Reverse Phase Chromatography

The main difference between normal phase and reverse phase chromatography is that normal phase chromatography has a very polar stationary phase and a non-polar mobile phase whereas reverse phase chromatography has a non-polar stationary phase and a polar mobile phase.

What is meant by reverse phase chromatography?

Reversed-phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase, which has a stronger affinity for hydrophobic or less polar compounds.

Is HPLC normal phase or reverse phase?

Reversed-Phase HPLC The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase.