What's a Chimeric Enzyme?

Chimeric restriction enzymes are a novel class of engineered nucleases in which the non-specific DNA cleavage domain of FokI (a type IIS restriction endonuclease) is fused to other DNA-binding motifs. ... Recently, one such chimeric nuclease, Zif-QQR-F_N was shown to find and cleave its target in vivo.

What do you mean by chimeric protein?

Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes which are originally coded for separate or same proteins. Translation of this fusion gene results in a single polypeptide with functional properties derived from each of the original proteins.

How do you make a chimeric protein?

Chimeric proteins can easily be prepared by recombinant means in vitro by fusing the structural genes of the proteins in question in a suitable expression vector. The translational 3′-terminus of the first gene is deleted, as is the promoter of the 5′-terminus of the second structural gene.