When Should Inoculating Instruments Be Flame Sterilized?
For Each Portion of the Streak (3 Total per Plate), Flame-Sterilize the Inoculating Loop Just Prior to Use. Also, Flame-Sterilize the Loop Just After the Final...
For each portion of the streak (3 total per plate), flame-sterilize the inoculating loop just prior to use. Also, flame-sterilize the loop just after the final streak is performed in order to prevent contamination of the bench surface and as a consideration to others in the lab who may later use the inoculating loops.
When should tubes be flamed?
Flaming should be routinely done when caps are removed from tubes during transfer of cultures. The purpose of flaming is not to sterilize, but to warm the tube and create warm air convection currents up and away from the opening.
When should inoculating instruments be sterilized by the incinerator?
Inoculating Loops for MALDI-TOF
That's because inoculation loops must be thoroughly sterilized (autoclaved) using a micro incinerator or bunsen burner for at least 5-10 seconds between every streak or sample. Working this way also means introducing an open flame into your otherwise still air environment.
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