When to Perform an Eluate?

The eluate is tested against a panel of reagent RBCs to determine if the antibody coating the patient's RBCs has specificity for a blood group antigen. If such an antibody is identified, antigen-negative RBCs can then be transfused.

What is eluate used for?

Eluates are useful in complicated antibody identification procedures, as well as in the investigation of positive direct antiglobulin tests and autoantibodies.

In what situation S would you use elution procedures to identify an antibody?

This procedure is most commonly used in identification of complicated antibodies, sometimes in the workup of transfusion reactions or hemolytic disease of the newborn, as well as in the workup of warm autoantibodies.