Where Do Substrates Bind?

The molecules that an enzyme works with are called substrates. The substrates bind to a region on the enzyme called the active site. There are two theories explaining the enzyme-substrate interaction. In the lock-and-key model, the active site of an enzyme is precisely shaped to hold specific substrates.

Where do the substrates attach?

In fact, whatever type of biological reaction you can think of, there is probably an enzyme to speed it up! The part of the enzyme where the substrate binds is called the active site (since that's where the catalytic “action” happens). A substrate enters the active site of the enzyme.

Do substrates bind to proteins?

Substrates binding takes place between the two domains, and stabilize a closed conformation of the proteins in which the domains are tightly packed together with the substrate buried at the interface.