Which Enzyme Formulates the Methionine Before Translation?

The addition of the formyl group to methionine is catalyzed by the enzyme methionyl-tRNA formyltransferase.

What prevents methionine from being inserted into the beginning and Formylmethionine in the interior?

What prevents methionine from being inserted into the beginning and formylmethionine in the interior? Different tRNAs and different factors are involved. Initiation requires IF-2, which recognizes fmet-tRNA^femt but not metRNA^fmet. Conversely, in elongation, EF-Tu recognizes metRNA^met but not fmet-tRNA^fmet.

Why is methionine removed after translation?

Here, the initiator tRNA molecule is shown binding after the small ribosomal subunit has assembled on the mRNA; the order in which this occurs is unique to prokaryotic cells. ... In both prokaryotes and eukaryotes, these proteins have the methionine removed, so that alanine becomes the N-terminal amino acid (Table 1).