Why Are Explants Sterilized?

Basically, explants sterilization is the process of making explants contamination free before establishment of cultures. It is important that the explants be free of any contaminants including endophytic prior to tissue culture without losing their biological activity [12,13].

Why is it necessary to sterilize explants in a plant tissue culture?

Sterilization of explants is a key step in any plant tissue culture work, as the removal of all microorganisms, including bacteria and fungi, is essential to attain successful initiation, growth and development of the cultured tissues in vitro, which otherwise would be overwhelmed by the contaminants [2].

What is purpose of surface sterilization of explants?

Surface sterilization of explant is a process which involves the immersion of explants into appropriate concentration of chemical sterilant(s) or disinfectant(s) for a specified time resulting in the establishment of a contamination-free culture.