Why Orcinol Is Used in Rna Estimation?
The Orcinol Reagent Reacts with Pentose Groups in the Backbone of the Rna Molecule and Depends on the Formation of Furfural, When the Pentose Is Heated with...
The orcinol reagent reacts with pentose groups in the backbone of the RNA molecule and depends on the formation of furfural, when the pentose is heated with concentrated hydrochloric acid. Orcinol reacts with the furfural in the presence of ferric chloride act as a catalyst to give a green colour.
How does orcinol help in RNA estimation?
Introduction: HiPer® RNA Estimation Teaching Kit is designed for rapid and accurate determination of RNA by orcinol reagent. This method depends on the conversion of pentose in the presence of hot acid to furfural which then reacts with orcinol to yield a green colour. The colour intensity can be measured at 665 nm.
Why is orcinol specific to RNA?
The standard orcinol test for estimating RNA is modified and developed as a specific method for the determination of RNA in the presence of DNA and proteins. ... 6H2O, and quantitation of RNA at its maximum absorbance under these conditions at 500 nm where interferences from DNA and proteins were minimal.
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