Why Pyrogen Test Is Essential in Parenteral Preparation?

Pyrogen test is performed to check the presence or absence of pyrogens in all aqueous parenterals. Rabbits are used to perform the test because their body temperature increases when pyrogen is introduced by the parenteral route.

Why are pyrogen required to be removed from parenteral products?

Pyrogen removal (depyrogenation) Pyrogens can often be difficult to remove from solution due to the high variability of their molecular weight. Pyrogens are also relatively thermally stable and insensitive to pH changes.

What is the significance of pyrogen in pharmaceutical preparations?

RADIOCHEMICAL METHODS | Pharmaceutical Applications

Bacterial endotoxins (pyrogens) are polysaccharides from bacterial membranes. They are water soluble, heat stable, and filterable. If they are present in a preparation and administered to a patient they can cause fever and also leukopenia in immunosuppressed patients.