Why Washed After Decalcification?

Decalcification may be hastened by increasing the proportion of Formic Acid to 25 ml; however, such concentration may make the solution opaque, thereby interfering with the staining result. 2. It requires neutralization with 5% sodium sulfate, and washing out to remove the acid from the tissue.

What is done during post decalcification?

Once the end-point has been determined and decalcification is complete, the tissue must be rinsed in cool tap water to remove excess decalcification solution. Some laboratories also use lithium carbonate to neutralize the remaining acid in tissue before processing.

How do you know when decalcification is complete?

Chemical method:

If solution is cloudy, tissue is still releasing calcium into decal solution. Decal solution should be changed and tissue should continue to decalcify. If solution is clear, decalcification is complete.