How Do You Get cDNA
A cDNA Probe Can Be Generated from a Specific mRNA. the mRNA, Encoding a Specific Protein, Is a Template. by the Action of Reverse Transcriptase and Dna...
A cDNA probe can be generated from a specific mRNA. The mRNA, encoding a specific protein, is a template. By the action of reverse transcriptase and DNA polymerase, a cDNA is formed that can be used as a probe to hybridize with a specific gene sequence (Fig. 10.45).
What is cDNA and how is it produced?
Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.
How do you get cDNA from the human genome?
- PMID: 8276268.
- DOI: 10.1016/0378-1119(93)90076-f.