How Do You Get cDNA

A cDNA probe can be generated from a specific mRNA. The mRNA, encoding a specific protein, is a template. By the action of reverse transcriptase and DNA polymerase, a cDNA is formed that can be used as a probe to hybridize with a specific gene sequence (Fig. 10.45).

What is cDNA and how is it produced?

Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.

How do you get cDNA from the human genome?

  1. PMID: 8276268.
  2. DOI: 10.1016/0378-1119(93)90076-f.
Sarah Jenkins

Sarah Jenkins

Senior Technology Editor & AI Specialist

Sarah Jenkins is a veteran tech journalist with over 12 years of experience covering artificial intelligence, mobile innovations, and digital ethics. Her insights have appeared in leading technology publications worldwide.

Share this article
Twitter Facebook Pinterest